PRAM1 antibody (C-Term)

Catalog No. ABIN184622

The Goat Polyclonal anti-PRAM1 antibody has been validated for WB and ELISA. It is suitable to detect PRAM1 in samples from Human.

Quick Overview for PRAM1 antibody (C-Term) (ABIN184622)

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRAM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PRAM1 Antibody

Binding Specificity: C-Term

Purpose: PRAM1

Sequence: DFCDPLENQP LPLGR

Cross-Reactivity: Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: DFCDPLENQPLPLGR

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 90-100 kDa band seen in Jurkat cell lysates. Recommended for use at 1-3 μg/mL. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79 kDa, Moog-Lutz et al., (J. Biol. Chem, J

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PRAM1

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Alternative Name: PRAM1

Background: PRAM-1, PRAM-1 protein, PML-RARA target gene encoding an Adaptor Molecule-1, PML-RARA regulated adaptor molecule 1, MGC39864, PML-RAR

Gene ID: 84106

NCBI Accession: NP_115528

Pathways: Regulation of Leukocyte Mediated Immunity