PPP2CB antibody (C-Term)

Catalog No. ABIN184646

The Goat Polyclonal anti-PPP2CB antibody is suitable to detect PPP2CB in samples from Human, Mouse, Rat and Pig. It has been validated for WB, ELISA and IHC.

Quick Overview for PPP2CB antibody (C-Term) (ABIN184646)

Target: PPP2CB (Protein Phosphatase 2, Catalytic Subunit, beta Isozyme (PPP2CB))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2CB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PPP2CB Antibody

Binding Specificity: C-Term

Purpose: PPP2CA / PPP2CB

Sequence: EPHVTRRTPD YFL

Specificity: Two variants NP_001009552.1 and NP_004147.1 represent identical protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EPHVTRRTPDYFL, from the C Terminus of the protein sequence according to NP_002706.1, NP_004147.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney and Thyroid. Recommended concentration: 10 μg/mL.

Western Blot: Approx 36 kDa band observed in Human Cerebellum and in Mouse, Rat and Pig Brain lysates (calculated MW of 35.6 kDa according to Human NP_002706.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPP2CB

Target: PPP2CB (Protein Phosphatase 2, Catalytic Subunit, beta Isozyme (PPP2CB))

Alternative Name: PPP2CA / PPP2CB

Background: PPP2CA, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform, PP2Ac, PP2CA, RP-C, PP2A-alpha, protein phosphatase 2, catalytic subunit, alpha isoform, replication protein C, serine/threonine protein phosphatase 2A, catalytic subunit, alph

Gene ID: 5515, 5516, 19052

NCBI Accession: NP_002706, NP_004147

Pathways: Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades