ATF7 antibody (C-Term)

Catalog No. ABIN184653

This anti-ATF7 antibody is a Goat Polyclonal antibody detecting ATF7 in WB and ELISA. Suitable for Human.

Quick Overview for ATF7 antibody (C-Term) (ABIN184653)

Target: ATF7 (Activating Transcription Factor 7 (ATF7))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ATF7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ATF7 Antibody

Binding Specificity: C-Term

Purpose: ATF7

Sequence: HVIMTPQSQS AGR

Specificity: This antibody is expected to recognise all three reported isoforms (NP_001123531.1, NP_006847.1 and NP_001123532.1).

Cross-Reactivity: Cow, Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HVIMTPQSQSAGR, from the C Terminus of the protein sequence according to NP_001123531.1, NP_006847.1, NP_001123532.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Heart lysates (calculated MW of 53.0 kDa according to NP_001123531.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATF7

Target: ATF7 (Activating Transcription Factor 7 (ATF7))

Alternative Name: ATF7

Background: ATF7, ATFA, activating transcription factor 7, MGC57182

Gene ID: 11016

NCBI Accession: NP_001123531, NP_006847, NP_001123532