PACSIN1 antibody (N-Term)

Catalog No. ABIN184665

This anti-PACSIN1 antibody is a Goat Polyclonal antibody detecting PACSIN1 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for PACSIN1 antibody (N-Term) (ABIN184665)

Target: PACSIN1 (Protein Kinase C and Casein Kinase Substrate in Neurons 1 (PACSIN1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PACSIN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PACSIN1 Antibody

Binding Specificity: N-Term

Purpose: PACSIN1

Sequence: SSSYDEASLA PEET

Specificity: Reported variants represent identical protein (NP_065855.1, NP_001186512.1).

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SSSYDEASLAPEET-C, from the N Terminus of the protein sequence according to NP_065855.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex and Cerebellum). Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 51.1 kDa according to NP_065855.1). Recommended concentration: 0.03-0.1 μg/mL. An additional band of unknown identity was also consistently observed at 37 kDa.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PACSIN1

Target: PACSIN1 (Protein Kinase C and Casein Kinase Substrate in Neurons 1 (PACSIN1))

Alternative Name: PACSIN1

Background: PACSIN1, protein kinase C and casein kinase substrate in neurons 1, KIAA1379, SDPI, syndapin I

Gene ID: 29993

NCBI Accession: NP_065855