DDAH1 antibody (C-Term)

Catalog No. ABIN184669

This Goat Polyclonal antibody specifically detects DDAH1 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for DDAH1 antibody (C-Term) (ABIN184669)

Target: DDAH1 (Dimethylarginine Dimethylaminohydrolase 1 (DDAH1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DDAH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-DDAH1 Antibody

Binding Specificity: C-Term

Purpose: DDAH1

Sequence: TCCSVLINKK VDS

Specificity: This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TCCSVLINKKVDS, from the C Terminus of the protein sequence according to NP_036269.1, NP_001127917.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in Human Cerebellum, Kidney and Testis lysates, and in lysates of cell lines HepG2 and LNCaP (calculated MW of 31.1 kDa according to NP_036269.1). This molecular weight is routinely observed by other sources. Rec

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Immunofluorescence: Strong expression of the protein seen in HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DDAH1

Target: DDAH1 (Dimethylarginine Dimethylaminohydrolase 1 (DDAH1))

Alternative Name: DDAH1

Background: DDAH1, dimethylarginine dimethylaminohydrolase 1, DDAH, DDAHI, NG, NG-dimethylarginine dimethylaminohydrolase, RP4-621F18.1, FLJ21264, FLJ25539

Gene ID: 23576

NCBI Accession: NP_036269, NP_001127917