CBL antibody (C-Term)

Catalog No. ABIN184673

The Goat Polyclonal anti-CBL antibody has been validated for WB and ELISA. It is suitable to detect CBL in samples from Mouse.

Quick Overview for CBL antibody (C-Term) (ABIN184673)

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CBL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CBL Antibody

Binding Specificity: C-Term

Purpose: CBL

Sequence: REFVSISSPA HVAT

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-REFVSISSPAHVAT, from the C Terminus of the protein sequence according to NP_005179.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 120 kDa band seen in 3T3 lysate. Recommended for use at 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CBL

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Alternative Name: CBL

Background: CBL, CBL2, C-CBL, RNF55, Cas-Br-M (murine) ecotropic retroviral transforming sequence, Cas-Br-M ecotropic retroviral transforming sequence (Oncogene CBL2), oncogene CBL2

Gene ID: 867, 12402

NCBI Accession: NP_005179

Pathways: TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals