ELMO1 antibody (C-Term)

Catalog No. ABIN184680

This anti-ELMO1 antibody is a Goat Polyclonal antibody detecting ELMO1 in WB and ELISA. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for ELMO1 antibody (C-Term) (ABIN184680)

Target: ELMO1 (Engulfment and Cell Motility 1 (ELMO1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ELMO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ELMO1 Antibody

Binding Specificity: C-Term

Purpose: ELMO1

Sequence: PKEPSNYDFV YDCN

Specificity: This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Zebrafish (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PKEPSNYDFVYDCN, from the C Terminus of the protein sequence according to NP_055615.8, NP_569709.1, NP_001034548.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 83 kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8 kDa according to NP_055615.8). Recommended concentration: 0.1-0.3 μg/mL. Primary inc

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunoprecipitation: A customer found positive results in IP on Human promyelocytic leukemia cells (HL60). This data is from a previous batch, not on sale.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ELMO1 antibody (ABIN184680)

Epting, Wendik, Bennewitz, Dietz, Driever, Kroll: "The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish." in: Circulation research, Vol. 107, Issue 1, pp. 45-55, (2010) (PubMed).

Target Details for ELMO1

Target: ELMO1 (Engulfment and Cell Motility 1 (ELMO1))

Alternative Name: ELMO1

Background: ELMO1, engulfment and cell motility 1 (ced-12 homolog, C. elegans), CED12, KIAA0281, ced-12 homolog 1, engulfment and cell motility 1, CED-12, ELMO-1, MGC126406, OTTHUMP00000207056, OTTHUMP00000207060

Gene ID: 9844, 140580

NCBI Accession: NP_055615, NP_569709, NP_001034548