ELMO2 antibody (C-Term)

Catalog No. ABIN184684

This Goat Polyclonal antibody specifically detects ELMO2 in WB, ELISA, IHC, FACS and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ELMO2 antibody (C-Term) (ABIN184684)

Target: ELMO2 (Engulfment and Cell Motility 2 (ELMO2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ELMO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-ELMO2 Antibody

Binding Specificity: C-Term

Purpose: ELMO2

Sequence: PKEPSSYDFV YHYG

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-PKEPSSYDFVYHYG

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows pixulated staining in the cytoplasm of stroma cells. Recommended concentration: 3-6 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell lines HEK293, Jurkat, RAW264.7 and in Human Cerebellum and Rat Brain lysates (calculated MW of 82.6 kDa according to Human NP_573403.1 and NP_877496.1 and Mouse NP_525026.2, and 83.7 kDa according

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in A549 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ELMO2

Target: ELMO2 (Engulfment and Cell Motility 2 (ELMO2))

Alternative Name: ELMO2

Background: ELMO2, engulfment and cell motility 2 (ced-12 homolog, C. elegans), CED12, FLJ11656, KIAA1834, ced-12 homolog 2, PH domain protein CED12A, engulfment and cell motility 2, engulfment and cell motility 2, RP11-394O2.2, CED-12, ELMO-2

Gene ID: 63916, 140579

NCBI Accession: NP_877496, NP_573403