DKK4 antibody (C-Term)

Catalog No. ABIN184710

This anti-DKK4 antibody is a Goat Polyclonal antibody detecting DKK4 in WB and ELISA. Suitable for Human.

Quick Overview for DKK4 antibody (C-Term) (ABIN184710)

Target: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DKK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DKK4 Antibody

Binding Specificity: C-Term

Purpose: DKK4

Sequence: RQHARLRVCQ KIEKL

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RQHARLRVCQKIEKL, from the C Terminus of the protein sequence according to NP_055235.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30-35 kDa band seen in Jurkat lysate. Recommended for use at 1-3 μg/mL. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa [form (i)], 30-32 kDa [form (ii)] and 15-17 kDa [f

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DKK4

Target: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Alternative Name: DKK4

Background: DKK4, dickkopf homolog 4 (Xenopus laevis), DKK-4, hdkk-4, Dickkopf gene 4, dickkopf (Xenopus laevis) homolog 4, MGC129562, MGC129563, dickkopf homolog 4

Gene ID: 27121

NCBI Accession: NP_055235

Pathways: WNT Signaling