ADAM17 antibody (C-Term)

Catalog No. ABIN184721

The Goat Polyclonal anti-ADAM17 antibody has been validated for WB and ELISA. It is suitable to detect ADAM17 in samples from Human.

Quick Overview for ADAM17 antibody (C-Term) (ABIN184721)

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ADAM17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ADAM17 Antibody

Binding Specificity: C-Term

Purpose: ADAM17 / TACE

Sequence: LQRQNRVDSK ETEC

Specificity: This antibody is expected to recognise isoform 1 (represented by NP_003174.3) and not isoform 2 (represented by NP_068604).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence LQRQNRVDSKETEC, from the C Terminus of the protein sequence according to NP_003174.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 125-130 kDa band observed in Hela, Jurkat and U937 lysates (predicted MW of kDa according to NP_003174.3 but glycosylated). Recommended for use at 0.1-1 μg/mL. Primary incubation was for 1 hour. The observed molecular weights correspo

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: ADAM17

Background: ADAM17, TACE, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), CSVP, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM metallopeptidase domain 17, TNF-alpha converting enzyme, a disintegrin and metalloproteinas

Gene ID: 6868, 11491

NCBI Accession: NP_003174

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus