KLF3 antibody (N-Term)

Catalog No. ABIN184727

The Goat Polyclonal anti-KLF3 antibody has been validated for WB, IF, ELISA and FACS. It is suitable to detect KLF3 in samples from Human.

Quick Overview for KLF3 antibody (N-Term) (ABIN184727)

Target: KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KLF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-KLF3 Antibody

Binding Specificity: N-Term

Purpose: KLF3 / BKLF

Sequence: LMFDPVPVKQ EAMD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence LMFDPVPVKQEAMD-C, from the N Terminus of the protein sequence according to NP_057615.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38-40 kDa band observed in Human Heart lysates (predicted MW of 38.8Da according to NP_057615.3). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KLF3

Target: KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))

Alternative Name: KLF3

Background: KLF3, BKLF, Kruppel-like factor 3 (basic), basic kruppel like factor, MGC48279

Gene ID: 51274

NCBI Accession: NP_057615