Septin 3 antibody (N-Term)

Catalog No. ABIN184733

The Goat Polyclonal anti-Septin 3 antibody has been validated for WB, ELISA and IHC. It is suitable to detect Septin 3 in samples from Human, Mouse and Rat.

Quick Overview for Septin 3 antibody (N-Term) (ABIN184733)

Target: Septin 3 (SEPT3)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Septin 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Septin 3 Antibody

Binding Specificity: N-Term

Purpose: Septin 3

Sequence: SELVPEPRPK PA

Specificity: This antibody is expected to recognize both reported isoforms according to NP_663786.2 and NP_061979.3.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SELVPEPRPKPA-C, from the N Terminus of the protein sequence according to NP_663786.1, NP_061979.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and (Cerebellum) and Testis. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in Human Cerebellum and in Mouse and Rat Brain lysates (calculated MW of 40.7 kDa according to Human NP_663786.2 and Rat NP_062248.2, and 40.6 kDa according to Mouse NP_001345765.1). Recommended concentration: 0.03-

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Septin 3

Target: Septin 3 (SEPT3)

Alternative Name: SEPT3

Background: SEPT3, septin 3, SEP3, CTA-250D10.3, MGC133218, bK250D10.3, OTTHUMP00000028742, neuronal-specific septin 3, G-Septin

Gene ID: 55964, 24050

NCBI Accession: NP_663786, NP_061979

Pathways: Negative Regulation of intrinsic apoptotic Signaling