Flotillin 1 antibody (C-Term)

Catalog No. ABIN184756

This Goat Polyclonal antibody specifically detects Flotillin 1 in WB, ELISA and IHC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Flotillin 1 antibody (C-Term) (ABIN184756)

Target: Flotillin 1 (FLOT1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Flotillin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Flotillin 1 Antibody

Binding Specificity: C-Term

Purpose: Flotillin 1

Sequence: SISQVNHKPL RTA

Specificity: This antibody is expected to recognize both reported isoforms (NP_005794.1, NP_001305804.1).

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-SISQVNHKPLRTA

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in lysates of cell line K562 and in preliminary testing of cell lysate MCF7 (calculated MW of 47.4 kDa according to NP_005794.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperatu

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Flotillin 1 antibody (ABIN184756)

Vassilieva, Gerner-Smidt, Ivanov, Nusrat: "Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G965-76, (2008) (PubMed).

Target Details for Flotillin 1

Target: Flotillin 1 (FLOT1)

Alternative Name: FLOT1

Background: FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae, DADB-118P11.2

Gene ID: 10211, 14251

NCBI Accession: NP_005794, NP_001305804