MAP4K1 antibody (N-Term)

Catalog No. ABIN184758

This anti-MAP4K1 antibody is a Goat Polyclonal antibody detecting MAP4K1 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for MAP4K1 antibody (N-Term) (ABIN184758)

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MAP4K1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MAP4K1 Antibody

Binding Specificity: N-Term

Purpose: HPK1 / MAP4K1

Sequence: DVVDPDIFNR DPR

Specificity: This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DVVDPDIFNRDPR-C, from the N Terminus of the protein sequence according to NP_001036065.1, NP_009112.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thymus. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 95-100 kDa band observed in Jurkat (predicted MW of 96 kDa according to NP_009112). Recommended for use at 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MAP4K1

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Alternative Name: MAP4K1

Background: MAP4K1, mitogen-activated protein kinase kinase kinase kinase 1, HPK1, hematopoietic progenitor kinase 1

Gene ID: 11184

NCBI Accession: NP_001036065, NP_009112

Pathways: TCR Signaling, Signaling of Hepatocyte Growth Factor Receptor