KIF4A antibody (C-Term)

Catalog No. ABIN184768

This Goat Polyclonal antibody specifically detects KIF4A in WB, ELISA, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for KIF4A antibody (C-Term) (ABIN184768)

Target: KIF4A (Kinesin Family Member 4A (KIF4A))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This KIF4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-KIF4A Antibody

Binding Specificity: C-Term

Purpose: KIF4A

Sequence: SFFSGCSPIE EEAH

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SFFSGCSPIEEEAH, from the C Terminus of the protein sequence according to NP_036442.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 139.5 kDa according to Mouse NP_032472.2). Recommended concentration:1-2 μg/mL. Primary incubation 1 hour at room temperature. Negative Control:

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KIF4A

Target: KIF4A (Kinesin Family Member 4A (KIF4A))

Alternative Name: KIF4A

Background: KIF4A, kinesin family member 4A, FLJ12530, FLJ12655, FLJ14204, FLJ20631, HSA271784, KIF4, KIF4-G1, chromokinesin, kinesin family member 4

NCBI Accession: NP_036442