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BAG3 antibody (C-Term)

This anti-BAG3 antibody is a Goat Polyclonal antibody detecting BAG3 in WB, ELISA and IHC. Suitable for Human.
Catalog No. ABIN184770

Quick Overview for BAG3 antibody (C-Term) (ABIN184770)

Target

See all BAG3 Antibodies
BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Reactivity

  • 61
  • 23
  • 16
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 64
  • 4
Goat

Clonality

  • 52
  • 16
Polyclonal

Conjugate

  • 33
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This BAG3 antibody is un-conjugated

Application

  • 43
  • 23
  • 19
  • 14
  • 13
  • 13
  • 6
  • 4
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade

Verified
  • Binding Specificity

    • 15
    • 7
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Purpose

    BAG3 / BIS/ CAIR1

    Sequence

    SSMTDTPGNP AAP

    Cross-Reactivity

    Human

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-SSMTDTPGNPAAP, from the C Terminus of the protein sequence according to NP_004272.2.

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 7 μg/mL.

    Western Blot: Approx 80-85 kDa band observed in lysates of cell line MCF7 (calculated MW of 61.6 kDa according to NP_004272.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 ho

    Peptide ELISA: antibody detection limit dilution 1:2000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    BAG3 (BCL2-Associated Athanogene 3 (BAG3))

    Alternative Name

    BAG3

    Background

    BAG3, BCL2-associated athanogene 3, BIS, BAG-3, CAIR-1, MGC104307, DKFZp434E0610, Bcl-2-binding protein, docking protein CAIR-1, BCL2-binding athanogene 3, BAG-family molecular chaperone regulator-3

    Gene ID

    9531

    NCBI Accession

    NP_004272
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