PAX8 antibody (N-Term)

Catalog No. ABIN184783

This Goat Polyclonal antibody specifically detects PAX8 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for PAX8 antibody (N-Term) (ABIN184783)

Target: PAX8 (Paired Box 8 (PAX8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PAX8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PAX8 Antibody

Binding Specificity: N-Term

Purpose: PAX8 (N Terminus)

Sequence: PHNSIRSGHG GLN

Specificity: This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PHNSIRSGHGGLN-C, from the N Terminus of the protein sequence according to NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney Cortex. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in Human Kidney lysates. An additional 48 kDa was also observed in some Kidney and HeLa cell lysates (calculated MW of 34.9 kDa according to Human NP_039247.1 PAX8D and 48.2 kDa according to Human NP_003457.1 PAXA).

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Alternative Name: PAX8

Background: PAX8, paired box gene 8, paired domain gene 8, paired box 8

Gene ID: 7849, 18510, 81819

NCBI Accession: NP_003457, NP_039245, NP_039246, NP_039247, NP_054698

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation