CBR1 antibody (C-Term)

Catalog No. ABIN184784

The Goat Polyclonal anti-CBR1 antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect CBR1 in samples from Human.

Quick Overview for CBR1 antibody (C-Term) (ABIN184784)

Target: CBR1 (Carbonyl Reductase 1 (CBR1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CBR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-CBR1 Antibody

Binding Specificity: C-Term

Purpose: CBR1

Sequence: HGQFVSEKRV EQW

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HGQFVSEKRVEQW, from the C Terminus of the protein sequence according to NP_001748.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 30-35 kDa band observed in Human Liver lysates (calculated MW of 30.4 kDa according to NP_001748.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CBR1

Target: CBR1 (Carbonyl Reductase 1 (CBR1))

Alternative Name: CBR1

Background: CBR1, carbonyl reductase 1, CBR, carbonyl reductase (NADPH), carbonyl reductase (NADPH) 1, hCBR1, prostaglandin 9-ketoreductase, prostaglandin-E(2) 9-reductase, SDR21C1, short chain dehydrogenase/reductase family 21C, member 1

Gene ID: 873

NCBI Accession: NP_001748