Importin 8 antibody (C-Term)

Catalog No. ABIN184793

The Goat Polyclonal anti-Importin 8 antibody has been validated for ELISA and IHC. It is suitable to detect Importin 8 in samples from Human.

Quick Overview for Importin 8 antibody (C-Term) (ABIN184793)

Target: Importin 8 (IPO8)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Importin 8 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Importin 8 Antibody

Binding Specificity: C-Term

Purpose: RANBP8 / IPO8

Sequence: SAFNFGTVPS NN

Specificity: This antibody is expected to recognise isoform 1 (NP_006381.2) and isoform 2 (NP_001177924.1).

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SAFNFGTVPSNN, from the C Terminus of the protein sequence according to NP_006381.2, NP_001177924.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Tonsil shows cytoplam / perinuclear staining in the parenchyma. Recommended concentration, 4-6 μg/mL.

Western Blot: Preliminary experiments in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1 μg/mL).

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Importin 8

Target: Importin 8 (IPO8)

Alternative Name: IPO8

Background: IPO8, importin 8, RANBP8, RAN-binding protein 8, FLJ26580, RAN binding protein 8

Gene ID: 10526

NCBI Accession: NP_006381, NP_001177924