FSTL1 antibody (C-Term)

Catalog No. ABIN184815

This anti-FSTL1 antibody is a Goat Polyclonal antibody detecting FSTL1 in WB, ELISA, FACS and IF. Suitable for Human.

Quick Overview for FSTL1 antibody (C-Term) (ABIN184815)

Target: FSTL1 (Follistatin-Like 1 (FSTL1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FSTL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-FSTL1 Antibody

Binding Specificity: C-Term

Purpose: FSTL1

Sequence: TAEKTKRVST KEI

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TAEKTKRVSTKEI, from the C Terminus of the protein sequence according to NP_009016.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band obseerved in Human Ovary lysates. (calculated MW of 35.0 kDa according to NP_009016.1). This band corresponds to earlier findings from other sources and was successfully blocked by incubation with the immunizing peptide. R

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and vesicles of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentrati

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FSTL1

Target: FSTL1 (Follistatin-Like 1 (FSTL1))

Alternative Name: FSTL1

Background: FSTL1, follistatin-like 1, FRP, FSL1, follistatin-related protein, FLJ50214, FLJ52277,

Gene ID: 11167

NCBI Accession: NP_009016