HMG20A antibody (C-Term)

Catalog No. ABIN184818

This anti-HMG20A antibody is a Goat Polyclonal antibody detecting HMG20A in WB, IHC and ELISA. Suitable for Human and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for HMG20A antibody (C-Term) (ABIN184818)

Target: HMG20A (High-Mobility Group 20A (HMG20A))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This HMG20A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-HMG20A Antibody

Binding Specificity: C-Term

Purpose: HMG20A

Sequence: ATVREVVNRL DR

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ATVREVVNRLDR, from the C Terminus of the protein sequence according to NP_060670.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows staining of nucleoli. Recommended concentration: 1-2 μg/mL.

Western Blot: This antibody has been successfully used in Rat Brain as described in the following paper: Artegiani et al, J Biol Chem. 2010 Aug 6,285(32):24740-50., PMID: 20530487.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-HMG20A antibody (ABIN184818)

Artegiani, Labbaye, Sferra, Quaranta, Torreri, Macchia, Ceccarini, Petrucci, Macioce: "The interaction with HMG20a/b proteins suggests a potential role for beta-dystrobrevin in neuronal differentiation." in: The Journal of biological chemistry, Vol. 285, Issue 32, pp. 24740-50, (2010) (PubMed).

Target Details for HMG20A

Target: HMG20A (High-Mobility Group 20A (HMG20A))

Alternative Name: HMG20A

Background: HMG20A, high-mobility group 20A, FLJ10739, HMGX1, HMGXB1, HMG box domain containing 1

Gene ID: 10363, 66867

NCBI Accession: NP_060670