SART1 antibody (N-Term)
Quick Overview for SART1 antibody (N-Term) (ABIN184888)
Target
See all SART1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- N-Term
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Purpose
- SART1
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Sequence
- GSSKKHRGEK EAA
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Specificity
- This antibody is expected to recognise both the human SART1(800) and SART1(259) proteins.
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Cross-Reactivity
- Cow, Human, Mouse, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence GSSKKHRGEKEAA-C, from the N Terminus of the protein sequence according to NP_005137.1.
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Isotype
- IgG
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Application Notes
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Western Blot: Approx 125 kDa band observed in HeLa lysates (consistent with Vertegaal et al, below, calculated MW of 90.3 kDa according to NP_005137). Recommended concentration: 0.03-0.1 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))
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Alternative Name
- SART1
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Background
- SART1, squamous cell carcinoma antigen recognised by T cells, ARA1, Ara1, HOMS1, MGC2038, SART1259, Snu66, IgE autoantigen, SART1(259) protein, SART1(800) protein, U4/U6.U5 tri-snRNP-associated 110 kDa protein, squamous cell carcinoma antigen recognised b
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Gene ID
- 9092, 20227, 29678
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NCBI Accession
- NP_005137
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Pathways
- Ribonucleoprotein Complex Subunit Organization
Target
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