ZIC5 antibody (C-Term)

Catalog No. ABIN184890

This anti-ZIC5 antibody is a Goat Polyclonal antibody detecting ZIC5 in ELISA and IHC. Suitable for Human.

Quick Overview for ZIC5 antibody (C-Term) (ABIN184890)

Target: ZIC5 (Zinc Family Member 5 (ZIC5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ZIC5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ZIC5 Antibody

Binding Specificity: C-Term

Purpose: ZIC5

Sequence: EIYGNPEVVR TIH

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EIYGNPEVVRTIH, from the C Terminus of the protein sequence according to NP_149123.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine, Skin and Prostate. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 45 kDa band in human brain lysates at 1 μg/mL. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ZIC5

Target: ZIC5 (Zinc Family Member 5 (ZIC5))

Alternative Name: ZIC5

Background: ZIC5, zinc finger protein of the cerebellum 5, zinc family member 5 protein, Zic family member 5 (odd-paired homolog, Drosophila)

Gene ID: 85416

NCBI Accession: NP_149123

Pathways: Tube Formation