UBE2S antibody (N-Term)
Quick Overview for UBE2S antibody (N-Term) (ABIN184893)
Target
See all UBE2S AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- N-Term
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Purpose
- E2-EPF / UBE2S
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Sequence
- NSNVENLPPH IIR
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Cross-Reactivity
- Cow, Dog, Human, Mouse, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence NSNVENLPPHIIR-C, from the N Terminus of the protein sequence according to NP_055316.2.
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Isotype
- IgG
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Application Notes
- Peptide ELISA: antibody detection limit dilution 1:32000.
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Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of A431 and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))
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Alternative Name
- UBE2S
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Background
- E2-EPF, ubiquitin carrier protein, ubiquitin-protein ligase, ubiquitin-conjugating enzyme E2-24 kD, UBE2S, ubiquitin-conjugating enzyme E2S, E2EPF, EPF5, ubiquitin carrier protein S, ubiquitin-protein ligase S
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Gene ID
- 27338, 77891
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NCBI Accession
- NP_055316
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Pathways
- Ubiquitin Proteasome Pathway
Target
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