UBE2F antibody (C-Term)

Catalog No. ABIN184898

The Goat Polyclonal anti-UBE2F antibody has been validated for ELISA. It is suitable to detect UBE2F in samples from Human.

Quick Overview for UBE2F antibody (C-Term) (ABIN184898)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-UBE2F Antibody

Binding Specificity: C-Term

Purpose: UBE2F/NEDD8-conjugating enzyme

Sequence: RNKVDDYIKR YAR

Specificity: This antibody is expected to recognise isoform 1 (NP_542409.1) only.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RNKVDDYIKRYAR, from the C Terminus of the protein sequence according to NP_542409.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave no signal but low background in Human Kidney and Liver lysates at up to 1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: UBE2F

Background: UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 conjugating enzyme, NEDD8-conjugating enzyme

Gene ID: 140739

NCBI Accession: NP_542409