BRDT antibody (C-Term)

Catalog No. ABIN184907

The Goat Polyclonal anti-BRDT antibody has been validated for ELISA. It is suitable to detect BRDT in samples from Human. There is 1 publication available.

Quick Overview for BRDT antibody (C-Term) (ABIN184907)

Target: BRDT (Bromodomain, Testis-Specific (BRDT))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BRDT antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-BRDT Antibody

Binding Specificity: C-Term

Purpose: BRD6 / BRDT

Sequence: QSDIMTMFEN NFD

Specificity: Variants (NP_001717.2, NP_997072.1) encode the same protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-QSDIMTMFENNFD

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 75 kDa band in human testis lysate after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 108 kDa a

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-BRDT antibody (ABIN184907)

Gill-Sharma, Choudhuri, Ansari, DSouza: "Putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones." in: Clinical epigenetics, Vol. 4, Issue 1, pp. 23, (2013) (PubMed).

Target Details for BRDT

Target: BRDT (Bromodomain, Testis-Specific (BRDT))

Alternative Name: BRDT

Background: BRDT, bromodomain, testis-specific, BRD6, OTTHUMP00000011809, OTTHUMP00000197577, cancer/testis antigen 9, testis-specific bromodomain protein

Gene ID: 676, 114642

NCBI Accession: NP_001717, NP_997072