RNF13 antibody (C-Term)

Catalog No. ABIN184935

This anti-RNF13 antibody is a Goat Polyclonal antibody detecting RNF13 in ELISA, WB, IHC and FACS. Suitable for Human.

Quick Overview for RNF13 antibody (C-Term) (ABIN184935)

Target: RNF13 (Ring Finger Protein 13 (RNF13))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF13 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-RNF13 Antibody

Binding Specificity: C-Term

Purpose: RNF13 / RZF (isoform 1 and 3)

Sequence: PNGERDYNIA NTV

Specificity: Reported variants represent identical protein (NP_009213.1, NP_899237.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PNGERDYNIANTV, from the C Terminus of the protein sequence according to NP_009213.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: This antibody stained islets but not in ducts and acini of Human normal pancreas. However, in pancreatic ductal adenocarcinoma staining was in the ducts and acini.

Western Blot: Approx. 42 kDa band observed in lysates of cell line A431 and in preliminary testing of Human Lung lysate, and approx. 45 kDa in lysates of cell line HeLa and in preliminary testing of U251 cell lysate (calculated MW of 42.8 kDa according to

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RNF13

Target: RNF13 (Ring Finger Protein 13 (RNF13))

Alternative Name: RNF13

Background: RNF13, ring finger protein 13, RZF, RING zinc finger protein, MGC13689, FLJ93817

Gene ID: 11342

NCBI Accession: NP_009213