STRADB antibody (C-Term)

Catalog No. ABIN184938

This anti-STRADB antibody is a Goat Polyclonal antibody detecting STRADB in WB, ELISA, IHC and IF. Suitable for Human.

Quick Overview for STRADB antibody (C-Term) (ABIN184938)

Target: STRADB (STE20-Related Kinase Adaptor beta (STRADB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This STRADB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-STRADB Antibody

Binding Specificity: C-Term

Purpose: ALS2CR2 / ILPIP

Sequence: CDFPDEKDSY WEF

Specificity: This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CDFPDEKDSYWEF, from the C Terminus of the protein sequence according to NP_061041.2, AAF71042.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Heart shows staining of myocardial fibres in transverse section. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 35 kDa band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0 kDa according to NP_061041.2 (alpha isoform) and 31.2 kDa according to AAF71042.1 (beta isoform). In transfected HEK293 transiently expressing ILPIP a

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U251 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for STRADB

Target: STRADB (STE20-Related Kinase Adaptor beta (STRADB))

Alternative Name: STRADB

Background: STRADB, ALS2CR2, ILPIP, PAPK, ILPIPA, CALS-21, PRO1038, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, ILP-interacting protein ILPIPA, STE20-related kinase adaptor beta, STRAD beta, pseudokinase ALS2CR2

Gene ID: 55437

NCBI Accession: NP_061041

Pathways: AMPK Signaling