TRIM3 antibody (N-Term)

Catalog No. ABIN184941

This anti-TRIM3 antibody is a Goat Polyclonal antibody detecting TRIM3 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for TRIM3 antibody (N-Term) (ABIN184941)

Target: TRIM3 (Tripartite Motif Containing 3 (TRIM3))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-TRIM3 Antibody

Binding Specificity: N-Term

Purpose: BERP / RNF22

Sequence: AKREDSPGPE VQP

Specificity: Both variants (NP_006449.2, NP_150594.2,) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence AKREDSPGPEVQP-C, from the N Terminus of the protein sequence according to NP_006449.2, NP_150594.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates and approx 85 kDa band seen in Mouse Brain (calculated MW of 80.8 kDa according to Human NP_006449.2, Mouse NP_001272799.1 and Rat NP_113974.1). Recommended concent

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRIM3

Target: TRIM3 (Tripartite Motif Containing 3 (TRIM3))

Alternative Name: TRIM3

Background: TRIM3, BERP, RNF22, TRIM3, tripartite motif-containing 3, HAC1, RNF97, ring finger protein 22, brain expressed ring finger, tripartite motif protein TRIM3, FLJ16135

Gene ID: 10612, 55992, 83616

NCBI Accession: NP_006449, NP_150594