TRIM63 antibody (N-Term)
Quick Overview for TRIM63 antibody (N-Term) (ABIN184950)
Target
See all TRIM63 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- N-Term
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Purpose
- MURF1 / TRIM63 (N Term)
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Sequence
- DYKSSLIQDG NPM
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Cross-Reactivity
- Cow, Dog, Human
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence DYKSSLIQDGNPM-C, from the N Terminus of the protein sequence according to NP_115977.2.
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Isotype
- IgG
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Application Notes
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Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section. Recommended concentration: 1.25 μg/mL.
Western Blot: Preliminary experiments gave an approx 60 kDa band in Human Skeletal Muscle lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated siz
Peptide ELISA: antibody detection limit dilution 1:32000.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- TRIM63 (Tripartite Motif Containing 63 (TRIM63))
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Alternative Name
- TRIM63
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Background
- TRIM63, MURF1, MURF-1, RNF28, ring finger protein 28, IRF, SMRZ, MURF2, FLJ32380, iris ring finger protein, muscle specific ring finger protein 2, striated muscle RING zinc finger protein, tripartite motif-containing 63, muscle specific ring finger protei
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Gene ID
- 84676
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NCBI Accession
- NP_115977
Target
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