PARK7/DJ1 antibody (C-Term)

Catalog No. ABIN184951

This Goat Polyclonal antibody specifically detects PARK7/DJ1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PARK7/DJ1 antibody (C-Term) (ABIN184951)

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PARK7/DJ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-PARK7/DJ1 Antibody

Binding Specificity: C-Term

Purpose: PARK7 / DJ-1

Sequence: AAQVKAPLVL KD

Specificity: Reported variants (NP_001116849.1, NP_009193.2) represent the same protein.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-AAQVKAPLVLKD, from the C Terminus of the protein sequence according to NP_001116849.1, NP_009193.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 7 μg/mL.

Western Blot: Approx 24 kDa band observed in lysates of cell lines HeLa and Jurkat and of Human Brain (Cerebellum, Hippocampus, Frontal Cortex) and rodent Brain lysates (calculated MW of 19.9 kDa according to NP_001116849.1 and of NP_009193.2). Recommended

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen around the nucleus. Dividing cells appear to show enhanced expression. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PARK7/DJ1

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Alternative Name: PARK7

Background: PARK7, DJ1, DJ-1, Parkinson disease (autosomal recessive, early onset) 7, oncogene DJ1, CTA-215D11.1, FLJ27376, FLJ34360, FLJ92274, OTTHUMP00000001350, OTTHUMP00000001351, Parkinson disease protein 7, protein DJ-1

Gene ID: 11315, 57320, 117287

NCBI Accession: NP_009193

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport