ANP32B antibody (C-Term)

Catalog No. ABIN184956

This anti-ANP32B antibody is a Goat Polyclonal antibody detecting ANP32B in WB, ELISA and IHC. Suitable for Human, Mouse, Rat and Pig.

Quick Overview for ANP32B antibody (C-Term) (ABIN184956)

Target: ANP32B (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member B (ANP32B))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ANP32B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ANP32B Antibody

Binding Specificity: C-Term

Purpose: SSP29 / ANP32B

Sequence: KRKRETDDEG EDD

Specificity: This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KRKRETDDEGEDD

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in lobular cells. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 28 kDa band observed in Human Tonsil lysates, and approx 30 kDa band observed in Rat and Pig Spleen lysates and in lysates of cell line NIH3T3 (calculated MW of 28.8 kDa according to Human NP_006392.1, 31.1 kDa according to Mouse NP_5709

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ANP32B

Target: ANP32B (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member B (ANP32B))

Alternative Name: ANP32B

Background: ANP32B, SSP29, APRIL, PHAPI2, acidic (leucine-rich) nuclear phosphoprotein 32 family, member B, acidic protein rich in leucines, OTTHUMP00000063770

Gene ID: 10541, 67628, 170724

NCBI Accession: NP_006392