EVC2 antibody (C-Term)

Catalog No. ABIN184972

This Goat Polyclonal antibody specifically detects EVC2 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for EVC2 antibody (C-Term) (ABIN184972)

Target: EVC2 (Ellis Van Creveld Syndrome 2 (EVC2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EVC2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-EVC2 Antibody

Binding Specificity: C-Term

Purpose: EVC2 / Limbin

Sequence: LNAKKAMRAL GMD

Specificity: This antibody is expected to recognise isoform 1 (NP_667338.3) and isoform 2 (NP_001159608.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LNAKKAMRALGMD, from the C Terminus of the protein sequence according to NP_667338.3, NP_001159608.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of the cytotrophoblasts. Recommended concentration, 2-4 μg/mL.

Western Blot: Consistent band observed in 293 and human heart at approx 75 kDa with 1 hour incubation of primary at 0.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EVC2

Target: EVC2 (Ellis Van Creveld Syndrome 2 (EVC2))

Alternative Name: EVC2

Background: EVC2, LBN, LIMBIN, Ellis van Creveld syndrome 2 (limbin), limbin

Gene ID: 132884

NCBI Accession: NP_667338, NP_001159608

Pathways: Hedgehog Signaling