CARD6 antibody (N-Term)

Catalog No. ABIN184977

This anti-CARD6 antibody is a Goat Polyclonal antibody detecting CARD6 in ELISA and IHC. Suitable for Human.

Quick Overview for CARD6 antibody (N-Term) (ABIN184977)

Target: CARD6 (Caspase Recruitment Domain Family, Member 6 (CARD6))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CARD6 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CARD6 Antibody

Binding Specificity: N-Term

Purpose: CARD6

Sequence: ATESTPSEII ERER

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ATESTPSEIIERER-C, from the N Terminus of the protein sequence according to NP_115976.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Pancreas shows variable staining of cytoplasm in acinar cells. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx. 45-50 kDa and 25-30 kDa bands observed in A549, Human Heart and Human Skeletal Muscle lysates at 1 μg of primary. Please note that the bands we observe are different from the predicted size of approx. 119 kDa according to NP_115976. Cur

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CARD6

Target: CARD6 (Caspase Recruitment Domain Family, Member 6 (CARD6))

Alternative Name: CARD6

Background: CARD6, caspase recruitment domain family, member 6, caspase recruitment domain protein 6, CINCIN1, CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation

Gene ID: 84674

NCBI Accession: NP_115976