PCGF2 antibody (C-Term)

Catalog No. ABIN184999

This Goat Polyclonal antibody specifically detects PCGF2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PCGF2 antibody (C-Term) (ABIN184999)

Target: PCGF2 (Polycomb Group Ring Finger 2 (PCGF2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PCGF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PCGF2 Antibody

Binding Specificity: C-Term

Purpose: MEL18 / PCGF2

Sequence: KMTVNGAPVP PLT

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KMTVNGAPVPPLT, from the C Terminus of the protein sequence according to NP_009075.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37-38 kDa band observed in lysates of cell lines A549 and PD19 (calculated MW of 37.8 kDa according to NP_009075.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PCGF2

Target: PCGF2 (Polycomb Group Ring Finger 2 (PCGF2))

Alternative Name: PCGF2

Background: PCGF2, MEL-18, polycomb group ring finger 2, RNF110, ZNF144, ring finger protein 110, zinc finger protein 144, MGC10545

Gene ID: 7703

NCBI Accession: NP_009075

Pathways: Chromatin Binding