FOXA2 antibody (C-Term)

Catalog No. ABIN185022

This anti-FOXA2 antibody is a Goat Polyclonal antibody detecting FOXA2 in WB, ELISA and IHC. Suitable for Human and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for FOXA2 antibody (C-Term) (ABIN185022)

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-FOXA2 Antibody

Binding Specificity: C-Term

Purpose: FOXA2 / HNF3B

Sequence: GVYSRPIMNS S

Specificity: This antibody is expected to recognise both reported isoforms: NP_068556.2, NP_710141.1

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GVYSRPIMNSS, from the C Terminus of the protein sequence according to NP_068556.2, NP_710141.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 4-8 μg/mL.

Western Blot: Approx 60 kDa band observed in Human Liver and Rat Lung lysates (calculated MW of 48.3 kDa according to Human NP_710141.1 and 48.5 kDa according to Rat NP_036875.1). The observed molecular weight corresponds to earlier findings in literature (

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-FOXA2 antibody (ABIN185022)

Nakamura, Moore, Negishi, Sueyoshi: "Nuclear pregnane X receptor cross-talk with FoxA2 to mediate drug-induced regulation of lipid metabolism in fasting mouse liver." in: The Journal of biological chemistry, Vol. 282, Issue 13, pp. 9768-76, (2007) (PubMed).

Target Details for FOXA2

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Alternative Name: FOXA2

Background: FOXA2, HNF3B, forkhead box A2, TCF3B, MGC19807, hepatic nuclear factor-3-beta, hepatocyte nuclear factor 3, beta

Gene ID: 3170, 15376, 25099

NCBI Accession: NP_068556, NP_710141

Pathways: Dopaminergic Neurogenesis, Regulation of Carbohydrate Metabolic Process