FOXB1 antibody (C-Term)

Catalog No. ABIN185027

The Goat Polyclonal anti-FOXB1 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect FOXB1 in samples from Human and Mouse.

Quick Overview for FOXB1 antibody (C-Term) (ABIN185027)

Target: FOXB1 (Forkhead Box B1 (FOXB1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-FOXB1 Antibody

Binding Specificity: C-Term

Purpose: FOXB1 / FKH5

Sequence: TSPASALHSV AVH

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TSPASALHSVAVH, from the C Terminus of the protein sequence according to NP_036314.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in nuclear lysates of cell line HepG2 (calculated MW of 35.0 kDa according to NP_036314.2. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunocytochemsitry: Anonymous customer found nuclear staining at lumbar levels of the Mouse Spinal Cord at embryonic day 12.5. Recommended concentration 0.1-0.3ug/ml.
Immunofluorescence: This antibody has been succe

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FOXB1

Target: FOXB1 (Forkhead Box B1 (FOXB1))

Alternative Name: FOXB1

Background: FOXB1, forkhead box B1, FKH5, HFKH-5

Gene ID: 27023, 64290

NCBI Accession: NP_036314