FOXL2 antibody (C-Term)

Catalog No. ABIN185040

This anti-FOXL2 antibody is a Goat Polyclonal antibody detecting FOXL2 in WB, ELISA and IHC. Suitable for Human and Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for FOXL2 antibody (C-Term) (ABIN185040)

Target: FOXL2 (Forkhead Box L2 (FOXL2))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-FOXL2 Antibody

Binding Specificity: C-Term

Purpose: FOXL2 / BPES

Sequence: DSKTGALHSR LDL

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DSKTGALHSRLDL, from the C Terminus of the protein sequence according to NP_075555.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Ovary. Recommended concentration: 8 μg/mL.

Western Blot: Approx. 50 kDa band observed in Mouse Ovary lysates and in preliminary testing of Human Uterus and Rat Ovary lysate (calculated MW of 38.9 kDa according to Mouse NP_036150.1). This molecular weight is routinely observed by other sources. Reco

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-FOXL2 antibody (ABIN185040)

Padua, Fox, Jiang, Morse, Tevosian: "Simultaneous gene deletion of gata4 and gata6 leads to early disruption of follicular development and germ cell loss in the murine ovary." in: Biology of reproduction, Vol. 91, Issue 1, pp. 24, (2014) (PubMed).

Blount, Schmidt, Justice, Vale, Fischer, Bilezikjian: "FoxL2 and Smad3 coordinately regulate follistatin gene transcription." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 7631-45, (2009) (PubMed).

Target Details for FOXL2

Target: FOXL2 (Forkhead Box L2 (FOXL2))

Alternative Name: FOXL2

Background: FOXL2, BPES, forkhead box L2, PFRK, BPES1, PINTO, forkhead transcription factor FOXL2, blepharophimosis, epicanthus inversus and ptosis, Blepharophimosis, epicanthus inversus, and ptosis 1, POF3

Gene ID: 668, 26927

NCBI Accession: NP_075555

Pathways: Nuclear Hormone Receptor Binding, Positive Regulation of Endopeptidase Activity