Coronin 1a antibody (C-Term)

Catalog No. ABIN185063

The Goat Polyclonal anti-Coronin 1a antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect Coronin 1a in samples from Human. There is 1 publication available.

Quick Overview for Coronin 1a antibody (C-Term) (ABIN185063)

Target: Coronin 1a (CORO1A) (Coronin, Actin Binding Protein, 1A (CORO1A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Coronin 1a antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Coronin 1a Antibody

Binding Specificity: C-Term

Purpose: Coronin 1 / TACO

Sequence: KRLDRLEETV QAK

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KRLDRLEETVQAK, from the C Terminus of the protein sequence according to NP_009005.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen and Small Intestine. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 60 kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 and approx. 55 kDa in Human Lynph node and Spleen lysates (calculated MW of 51.0 kDa according to Human NP_009005.1). These molecular weights are routinely observed

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Coronin 1a antibody (ABIN185063)

Honoré, Buus, Claësson: "Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53." in: Proteome science, Vol. 6, pp. 18, (2008) (PubMed).

Target Details for Coronin 1a

Target: Coronin 1a (CORO1A) (Coronin, Actin Binding Protein, 1A (CORO1A))

Alternative Name: CORO1A

Background: CORO1A, Coronin 1, TACO, p57, CLABP, HCORO1, CLIPINA, Clabp TACO, coronin, actin binding protein, 1A, coronin-1, coronin, actin-binding, 1A, coronin, actin-binding protein, 1A, FLJ41407, MGC117380,

Gene ID: 11151, 12721, 155151

NCBI Accession: NP_009005

Pathways: Regulation of Actin Filament Polymerization