FBXW2 antibody (C-Term)

Catalog No. ABIN185067

This anti-FBXW2 antibody is a Goat Polyclonal antibody detecting FBXW2 in ELISA, WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for FBXW2 antibody (C-Term) (ABIN185067)

Target: FBXW2 (F-Box and WD Repeat Domain Containing 2 (FBXW2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This FBXW2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-FBXW2 Antibody

Binding Specificity: C-Term

Purpose: FBXW2

Sequence: KRGSSFLAGE HPG

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KRGSSFLAGEHPG, from the C Terminus of the protein sequence according to NP_036296.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3 μg/mL. An anonymous customer found positive results in IHC on Mouse kidney tissue. An anonymous c

Western Blot: Approx 48 kDa band observed in Human Kidney lysates (calculated MW of 48.0 kDa according to NP_036296.1). Recommended concentration: 0.3-1.5 μg/mL. A stronger band of unknown identity was also consistently observed at appr. 30 kDa - both band

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FBXW2

Target: FBXW2 (F-Box and WD Repeat Domain Containing 2 (FBXW2))

Alternative Name: FBXW2

Background: FBXW2, F-box and WD-40 domain protein 2, FBW2, Fwd2, MGC117371, Md6, F-box protein Fbw2, F-box and WD repeat domain containing 2

Gene ID: 26190

NCBI Accession: NP_036296