BOLL antibody (C-Term)

Catalog No. ABIN185072

The Goat Polyclonal anti-BOLL antibody has been validated for ELISA and IHC. It is suitable to detect BOLL in samples from Human.

Quick Overview for BOLL antibody (C-Term) (ABIN185072)

Target: BOLL (Bol, Boule-Like (BOLL))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BOLL antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-BOLL Antibody

Binding Specificity: C-Term

Purpose: BOULE / BOLL

Sequence: QPEPIKTVWS IHY

Specificity: This antibody is expected to recognize both reported isoforms (NP_932074.1, NP_149019.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QPEPIKTVWSIHY, from the C Terminus of the protein sequence according to NP_932074.1, NP_149019.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. Recommended concentration, 4-6 μg/mL.

Western Blot: Preliminary experiments gave an approx 22 kDa band in Human Testis lysates after 1.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BOLL

Target: BOLL (Bol, Boule-Like (BOLL))

Alternative Name: BOLL

Background: BOLL, bol, boule-like (Drosophila), BOULE, boule

Gene ID: 66037

NCBI Accession: NP_932074, NP_149019