EPS8 antibody (C-Term)

Catalog No. ABIN185076

This anti-EPS8 antibody is a Goat Polyclonal antibody detecting EPS8 in WB, ELISA and IHC. Suitable for Human and Mouse.

Quick Overview for EPS8 antibody (C-Term) (ABIN185076)

Target: EPS8 (Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This EPS8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-EPS8 Antibody

Binding Specificity: C-Term

Purpose: EPS8

Sequence: SGVESFDEGS SH

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-SGVESFDEGSSH

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney and Pancreas. Recommended concentration: 6-8 μg/mL.

Western Blot: Approx. 100 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 91.9 kDa according to NP_004438.3). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 70 kDa. This band

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EPS8

Target: EPS8 (Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8))

Alternative Name: EPS8

Background: EPS8, epidermal growth factor receptor pathway substrate 8

Gene ID: 2059, 13860

NCBI Accession: NP_004438

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization