CSTB antibody (C-Term)

Catalog No. ABIN185101

This Goat Polyclonal antibody specifically detects CSTB in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for CSTB antibody (C-Term) (ABIN185101)

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CSTB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CSTB Antibody

Binding Specificity: C-Term

Purpose: Cystatin B / Stefin B

Sequence: QTNKAKHDEL TYF

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QTNKAKHDELTYF, from the C Terminus of the protein sequence according to NP_000091.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 6-7 μg/mL.

Western Blot: Approx 16 kDa band observed in Human Liver, Lung and Kidney Lysates (calculated MW of 11.1 kDa according to NP_000091.1 ). The observed molecular weight corresponds to earlier findings in literature with with different antibodies (Lee et al,

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CSTB

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Alternative Name: CSTB

Background: CSTB, cystatin B (stefin B), PME, CST6, STFB, CPI-B, stefin B, liver thiol proteinase inhibitor, EPM1, cystatin B

Gene ID: 1476

NCBI Accession: NP_000091

Pathways: Response to Water Deprivation