FARP1 antibody (FERM, RhoGEF (ARHGEF) and Pleckstrin Domain Protein 1 (Chondrocyte-Derived)) (N-Term)

Details for Product anti-FARP1 Antibody No. ABIN185104
Target
Binding Specificity
N-Term
8
8
7
7
4
3
1
1
Reactivity
Human
31
Host
Goat
21
6
4
Clonality
Polyclonal
Conjugate
This FARP1 antibody is un-conjugated
4
4
4
3
3
3
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
27
22
3
1
1
Options
Purpose CDEP / FARP1
Immunogen Peptide with sequence GEIEQRPTPGSRL-C, from the N Terminus of the protein sequence according to NP_005757.1, NP_001001715.2.
Blocking Peptide Blocking peptide for this product available: ABIN370146
Sequence GEIEQRPTPG SRL
Isotype IgG
Specificity This antibody is expected to recognise isoform 1 (NP_005757.1) and isoform 2 (NP_001001715.2).
Predicted Reactivity Human, Mouse, Rat, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name FARP1 (FARP1 Antibody Abstract)
Background FARP1, FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived), CDEP, chondrocyte-derived ezrin-like protein, RP11-111L24.1, MGC87400, PLEKH2, FERM, RhoGEF, and pleckstrin domain protein 1
Gene ID 10160
NCBI Accession NP_005757, NP_001001715, NP_001273768
Application Notes

DS WB Results: Preliminary experiments showed an approx 110-120 kDa band in Human Frontal Cortex lysates (calculated MW of 119 kDa according to NP_ 005757.1).. An additional band was also consistently observed at 40 kDa and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-FERM, RhoGEF (ARHGEF) and Pleckstrin Domain Protein 1 (Chondrocyte-Derived) (FARP1) (N-Term) antibody (ABIN185104) ABIN185104 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabi...
Image no. 2 for anti-FERM, RhoGEF (ARHGEF) and Pleckstrin Domain Protein 1 (Chondrocyte-Derived) (FARP1) (N-Term) antibody (ABIN185104) ABIN185104 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabi...
Image no. 3 for anti-FERM, RhoGEF (ARHGEF) and Pleckstrin Domain Protein 1 (Chondrocyte-Derived) (FARP1) (N-Term) antibody (ABIN185104) ABIN185104 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line)...