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DS WB Results: Preliminary experiments showed an approx 110-120 kDa band in Human Frontal Cortex lysates (calculated MW of 119 kDa according to NP_ 005757.1).. An additional band was also consistently observed at 40 kDa and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.