STXBP1 antibody (C-Term)

Catalog No. ABIN185107

This Goat Polyclonal antibody specifically detects STXBP1 in WB, ELISA and IHC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for STXBP1 antibody (C-Term) (ABIN185107)

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This STXBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-STXBP1 Antibody

Binding Specificity: C-Term

Purpose: MUNC18 / STXBP1 (isoform a)

Sequence: SRVSFEDQAP TME

Specificity: This antibody is expected to recognize isoform a (NP_003156.1).

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SRVSFEDQAPTME, from the C Terminus of the protein sequence according to NP_003156.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 70-75 kDa band observed in Human Cerebellum lysates (calculated MW of 68.7 kDa according to Human NP_003156.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-STXBP1 antibody (ABIN185107)

Meijer, Cijsouw, Toonen, Verhage: "Synaptic Effects of Munc18-1 Alternative Splicing in Excitatory Hippocampal Neurons." in: PLoS ONE, Vol. 10, Issue 9, pp. e0138950, (2016) (PubMed).

Target Details for STXBP1

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Alternative Name: STXBP1

Background: STXBP1, MUNC18-1, UNC18, hUNC18, syntaxin binding protein 1, Hunc18, rbSec1, syntaxin-binding protein 1, RP11-56D16.3, EIEE4, FLJ37475, RBSEC1

Gene ID: 6812, 20910

NCBI Accession: NP_003156

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport