VPS45 antibody (C-Term)

Catalog No. ABIN185111

The Goat Polyclonal anti-VPS45 antibody has been validated for ELISA, WB and IHC. It is suitable to detect VPS45 in samples from Human.

Quick Overview for VPS45 antibody (C-Term) (ABIN185111)

Target: VPS45 (Vacuolar Protein Sorting 45 (VPS45))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS45 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-VPS45 Antibody

Binding Specificity: C-Term

Purpose: VPS45 (C Terminus)

Sequence: ESSQVTSRSA SRR

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ESSQVTSRSASRR, from the C Terminus of the protein sequence according to NP_009190.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex), Placenta and Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Human Testis and Peripheral Blood Mononucleocyte lysates (calculated MW of 65.1 kDa according to NP_009190.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VPS45

Target: VPS45 (Vacuolar Protein Sorting 45 (VPS45))

Alternative Name: VPS45

Background: VPS45, VPS45A, VPS45B, VSP45, VSP45A, H1, H1VPS45, leucocyte vacuolar protein sorting 45, vacuolar protein sorting 45A, vacuolar protein sorting 45A (yeast homolog), vacuolar protein sorting 45 homolog (S. cerevisiae), vacuolar protein sorting 45A (yeast)

Gene ID: 11311, 22365

NCBI Accession: NP_009190

Pathways: Transition Metal Ion Homeostasis, Synaptic Vesicle Exocytosis