CYP7B1 antibody (C-Term)

Catalog No. ABIN185122

This Goat Polyclonal antibody specifically detects CYP7B1 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CYP7B1 antibody (C-Term) (ABIN185122)

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CYP7B1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CYP7B1 Antibody

Binding Specificity: C-Term

Purpose: CYP7B1

Sequence: YPDSDVLFRY KVKS

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YPDSDVLFRYKVKS, from the C Terminus of the protein sequence according to NP_004811.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast shows textured cytoplasm staining in ductal epithelial cells. Recommended concentration, 3-6 μg/mL.

Western Blot: Preliminary experiments in Human Liver, Kidney and Prostate lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been repor

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYP7B1

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Alternative Name: CYP7B1

Background: CYP7B1, CP7B, cytochrome P450, family 7, subfamily B, polypeptide 1, oxysterol 7alpha-hydroxylase, cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1, CBAS3, SPG5A

Gene ID: 9420

NCBI Accession: NP_004811

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Intracellular Steroid Hormone Receptor Signaling