ARHGEF18 antibody (C-Term)

Catalog No. ABIN185123

The Goat Polyclonal anti-ARHGEF18 antibody has been validated for ELISA, WB and IF. It is suitable to detect ARHGEF18 in samples from Human. There are 4+ publications available.

Quick Overview for ARHGEF18 antibody (C-Term) (ABIN185123)

Target: ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ARHGEF18 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Grade: Verified

Product Details anti-ARHGEF18 Antibody

Binding Specificity: C-Term

Purpose: P114-RHO-GEF / ARHGEF18

Sequence: SAKEDASKED VIFF

Specificity: This antibody is expected to recognize both reported isoforms (NP_056133.2, NP_001124427.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2, NP_001124427.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 140+90 kDa band observed in lysates of cell line Daudi and Caco-2 (calculated MW of 114 kDa according to NP_056133.2 and 131 kDa accoeding to NP_001124427.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This anti

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 36404918.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ARHGEF18 antibody (ABIN185123)

Zaritsky, Tseng, Rabadán, Krishna, Overholtzer, Danuser, Hall: "Diverse roles of guanine nucleotide exchange factors in regulating collective cell migration." in: The Journal of cell biology, Vol. 216, Issue 6, pp. 1543-1556, (2017) (PubMed).

Herder, Swiercz, Müller, Peravali, Quiring, Offermanns, Wittbrodt, Loosli: "ArhGEF18 regulates RhoA-Rock2 signaling to maintain neuro-epithelial apico-basal polarity and proliferation." in: Development (Cambridge, England), Vol. 140, Issue 13, pp. 2787-97, (2013) (PubMed).

Terry, Elbediwy, Zihni, Harris, Bailly, Charras, Balda, Matter: "Stimulation of cortical myosin phosphorylation by p114RhoGEF drives cell migration and tumor cell invasion." in: PLoS ONE, Vol. 7, Issue 11, pp. e50188, (2012) (PubMed).

Terry, Zihni, Elbediwy, Vitiello, Leefa Chong San, Balda, Matter: "Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis." in: Nature cell biology, Vol. 13, Issue 2, pp. 159-66, (2011) (PubMed).

Target Details for ARHGEF18

Target: ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))

Alternative Name: ARHGEF18

Background: ARHGEF18, Rho/Rac guanine nucleotide exchange factor (GEF) 18, P114-RHO-GEF, KIAA0521, MGC15913, Rho-specific guanine nucleotide exchange factor p114, P114-RhoGEF, Rho/Rac guanine nucleotide exchange factor 18

Gene ID: 23370

NCBI Accession: NP_056133, NP_001124427

Pathways: Neurotrophin Signaling Pathway