NUP85 antibody (C-Term)

Catalog No. ABIN185140

This Goat Polyclonal antibody specifically detects NUP85 in WB and ELISA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for NUP85 antibody (C-Term) (ABIN185140)

Target: NUP85 (Nucleoporin 85kDa (NUP85))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NUP85 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NUP85 Antibody

Binding Specificity: C-Term

Purpose: Pericentrin 1 / NUP85

Sequence: ARAIIREGSL EGS

Specificity: Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-ARAIIREGSLEGS

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in HeLa lysates (calculated MW of 75 kDa according to NP_079120.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-NUP85 antibody (ABIN185140)

Toda, Terashima, Sato, Hirose, Kanegasaki, Matsushima: "FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 10, pp. 6387-94, (2009) (PubMed).

Target Details for NUP85

Target: NUP85 (Nucleoporin 85kDa (NUP85))

Alternative Name: NUP85

Background: NUP85, pericentrin 1, PCNT1, PCN, PCNT, frount, nucleoporin Nup75, nucleoporin 85 kDa, FLJ12549, Nup75, nucleoporin 85

Gene ID: 79902, 18541

NCBI Accession: NP_079120