KPNA3 antibody (C-Term)

Catalog No. ABIN185159

The Goat Polyclonal anti-KPNA3 antibody has been validated for WB, IHC, ELISA, IF and FACS. It is suitable to detect KPNA3 in samples from Human. There are 3+ publications available.

Quick Overview for KPNA3 antibody (C-Term) (ABIN185159)

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KPNA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-KPNA3 Antibody

Binding Specificity: C-Term

Purpose: KPNA3 / IPOA4

Sequence: DPTANLQTKE FNF

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DPTANLQTKEFNF, from the C Terminus of the protein sequence according to NP_002258.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 6 μg/mL.

Western Blot: Approx. 60 kDa band observed in lysates of cell lines CaCo-2, HEK293 and MCF7 (calculated MW of 57.8 kDa according to NP_002258.2). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-KPNA3 antibody (ABIN185159)

Jovičić, Mertens, Boeynaems, Bogaert, Chai, Yamada, Paul, Sun, Herdy, Bieri, Kramer, Gage, Van Den Bosch, Robberecht, Gitler: "Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS." in: Nature neuroscience, Vol. 18, Issue 9, pp. 1226-9, (2015) (PubMed).

Ohara, Hata, Yasuhara, Mehmood, Yoneda, Nakagawa, Yamashita: "Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin ?." in: Biochemical and biophysical research communications, Vol. 405, Issue 4, pp. 697-702, (2011) (PubMed).

Huenniger, Krämer, Soom, Chang, Köhler, Depping, Kehlenbach, Kaether: "Notch1 signaling is mediated by importins alpha 3, 4, and 7." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 18, pp. 3187-96, (2010) (PubMed).

Target Details for KPNA3

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Alternative Name: KPNA3

Background: KPNA3, IPOA4, karyopherin alpha 3 (importin alpha 4), RP11-432M24.3, SRP1gamma, SRP4, hSRP1, importin alpha 4, importin alpha 4, importin-alpha-Q2, karyopherin alpha 3, SRP1, importin alpha-3

Gene ID: 3839, 16648

NCBI Accession: NP_002258

Pathways: Protein targeting to Nucleus